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Although the well-known antibiotic norfloxacin NOR is recognized as an important environmental pollutant, little is known about its impacts on ecological processes, particularly on species interactions. In h-long lethal experiment, mortality rates of D.
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Moreover, the growth of zooplankton individuals can be greatly inhibited by NOR 5, 9. Surprisingly, few studies have explicitly examined the role of NOR in phytoplankton-zooplankton interactions, which is required to understand the consequences of NOR contamination on ecological processes. NOR may affect phytoplankton-zooplankton interactions e. Zooplankton activity is highly sensitive to a wide range of environmental pollutants, including antibiotics, heavy metals, pesticides, and herbicides 10, In response to environmental pollutants, zooplankton individuals may increase their swimming ability i.
In addition, the swimming pattern e. The mechanism might be of relevance to the affected encounter rate between predator and its prey, assuming that encounter rate is a function of the swimming ability and body size of both predator and prey species Unfortunately, the impact and mechanism by which NOR might affect phytoplankton-zooplankton relationships is still largely unknown 2. The present study was designed to test if NOR affects phytoplankton-zooplankton interactions, based on the feeding rate of zooplankton on phytoplankton.
Thus, we conducted four separate experiments to examine Daphnia magna lethal mortality rate and sublethal heartbeat rate, behavior and feeding rate responses to NOR in the absence or presence of its prey, alga Chlorella pyrenoidosa. We hypothesized that: 1 mortality rate will gradually increase with increasing NOR concentration and exposure time, and 2 heartbeat rate, average swimming ability and feeding rate will change after NOR exposure; a positive correlation is also expected between average swimming ability and feeding rate.
The mortality rate of Daphnia magna was significantly affected by norfloxacin NOR concentration, exposure time, and their interactions in the absence of the green alga Chlorella pyrenoidosa Table 1, Fig. Additionally, at a given NOR concentration, mortality rate of D. Correspondently, the, and h LC 50 were Figure 1 was produced in Microsoft Excel Along with increasing NOR concentration, heartbeat rate reduced by 6. Figure 2 was produced in Microsoft Excel Swimming behavior in the presence of C.
Additionally, heartbeat rate and TVH Fig. Figure 3 was produced in Microsoft Excel Figure 4 was produced in Microsoft Excel In the absence of grazers, C. Moreover, the feeding rate was positively associated with average swimming ability in this alga-grazer system, regardless of experimental time Fig. Differences between algal density of C. Differences between mean feeding rates of D.
Figure 5 was produced in Microsoft Excel Figure 6 was produced in Microsoft Excel Although NOR is increasingly used in clinical treatments, its behavior and biological consequences when released into aquatic environments are still poorly understood. According to the results obtained in the present study, high NOR concentrations led to D. In our lethal experiment, higher NOR concentrations and longer exposure times increased mortality rates.
Moreover, we found that the magnitude of the contamination was a more important determinant for NOR toxicity to D. In sublethal experiments, NOR contamination led to a significantly decrease in heartbeat rate of D. Heartbeat rate has been used as an indicator of D. Vertical swimming represents an additional energy cost in relation to horizontal swimming as D.
Besides, there are trade-offs between the adaptation to specific stress and movement, such as increasing the tolerance to a specific stressor through maintaining movement at a low level 17, Consequently, the duration of quiescence significantly increased with increasing TVH, which finally led to a decrease in average swimming ability. Numerous studies have identified decreases in swimming ability of zooplankton individuals when exposed to environmental contaminants.
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Authored by Seth M. Weinreb, MD, FACS